Protocols

   
 

35S In Situ [pdf]

Beta-galactosidase assay [pdf]

Clean Wizard Prep [pdf]

Culture in Collagen Gels [pdf]

Developing 35S In Situ Slides [pdf]

DIG RNA Probe Synthesis [pdf]

Dirty Miniprep [pdf]

ECL Western [pdf]

Embedding, Sectioning, and Mounting of Frozen Tissues [pdf]

Filter Colony Transfer [pdf]

Filter Lysis [pdf]

Fresh Section DIG In Situ [pdf]

Genotyping from mouse tails [pdf]

Immunohistochemistry [pdf]

In Situ on Frozen Sections [pdf]

Info on using the Axiocam and Openlab software [pdf]

SHH-N Conditioned Media (CM) [pdf]

TRIzol method for RNA isolation [pdf]

Using the Maconnell Miniprep Machine [pdf]

Whole mount In Situ Protocol [pdf]